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Experimental Design and Relatedness Among Samples. The top portion of the figure depicts the family structure among the lines used for both the genomic and transcriptomic data in this study. Note that BTx3197 is a direct progenitor of both BTx623 and PR22. Orange colored stalks indicate non-sweet, dry stems, while blue stalks indicate sweet and juicy stems. Green colored stalks are intermediate. Bold type denotes lines with a publicly available reference genome. Short read Illumina re-sequencing was performed on any genotype with an asterisk (*) by its name. Boxes show which lines were used in the RNA-seq experiments. The lower portion of the figure shows the 5 time stages and 3 tissues collected at each time point for the RNA-seq study. All images used in this figure were originally created by E. Cooper for this manuscript 2b1af7f3a8